2.2. Preparation of the extract
Leaves plant extract was prepared by Soxhlet extraction
method. About 100 g of powder material was uniformly
packed into a thimble and run in Soxhlet extractor. It
was exhaustible extracted with methanol for the period
of about 48 h or 22 cycles or till the solvent in the siphon
tube of an extractor become color less. After that extracts
were filtered with the help of filter paper and solvent was
evaporated from extract in rotary evaporator to get the
syrupy consistency. Then extract w