The free glycerol content of the permeate was determined by volumetric method based on the official AOCS methodology for the analysis of free glycerol in oils and fats (Ca 14-56) [32]. Each sample was analyzed three times and the averages are reported. The titration method with sodium periodate allows fast and accurate determination of glycerol in oils and fats at a low cost compared with chromatographic methods [9,32,33]. When glycerol concentration in the permeate was too high, i.e., when there was permeation of the dispersed phase through the membrane, titration method could not be applied and it was only performed a volumetric quantification of the percentage of the dispersed phase containing glycerol.