For antioxidant activity test, a 100 PL sample, 100 PL 2(N-morpholino)ethanesulfonic acid (MES) buffer pH 7.4, and 100 PL DPPH (11.8 mg DPPH in 100 mL ethanol) were added in each well of a 96 well plate. After 30 min, the absorbance of the mixture was measured at 514 nm.
The inhibitory activity was calculated.