Asimple, reliable medium for pollen germination
of Cajanus cajan was developed by modifying
Brewbaker and Kwack BK) medium. Past attempts
ofC.cajanpollengermination inartificialmediawere
not successful. Amedium containing polyethylene
glycol 4000 PEG) showed more than 90% germina-
tion for C. cajan var. Pusa 33 only when the young
buds 36 h before anthesis) were kept in pollen
germination medium PGM) for 36h before pollen
extraction. Supplementation of PGM with «-amino
caproicacidEACA),anaminoacid,showedimproved
pollen germination in Pusa 33 and also helped to
avoid preconditioning of young buds before pollen
extraction. Itwasalsoobserved thatthereisageno-
typic difference in the level of EACA required for
invitro pollen germination. Thus acomplete medium
for C. cajan genotypes consists of 37.5% sucroseq