A voucher specimen is
kept in the laboratory and one set of specimen is submitted to
BARO herbarium, Department of Botany, The Maharaja Sayajirao
University of Baroda, Vadodara for future reference (KSR/38/2012).
After 12 h of
fixation, samples were transferred to 70% alcohol for
further storage and
fixation. Stem segments were trimmed to
suitable size (i.e. 2–3 mm) and dehydrated in tertiary butyl alcohol
series and embedded in paraffin after infiltration (Berlyn and
Miksche, 1976).