Fractions containing the His6-tagged protein were pooled, dialyzed against phosphate buffer (50 mM, pH 7) containing 300 mM NaCl, and analyzed for esterase activity.
Fractions containing the His6-tagged protein were pooled, dialyzed against phosphate buffer(50 mM, pH 7) containing 300 mM NaCl, and analyzed for esterase activity.