Some of them produced a small number of pycnidia on potato dextrose agar after incubation for 30 days. Artificial inoculation on wounded leaves of durian seedlings, resulted in the production of browning areas with yellow halos and pycnidium formation at 13 days and 20 days after inoculation, respectively. Furthermore, red-brown spots with yellow halos on leaf tissues were observed at 32 days after inoculation.