Alaska pollock whole fish (700 ± 50 g), caught in the East Bering
Sea on Feb 9, 2015, were randomly collected immediately after
offloading. They were subsequently frozen in a laminated box
(48×25.5×6 cm3
) using a plate freezer within 3 h postmortem.
Within 2½ h, the temperature of frozen whole fish reached −20 °C.
Three frozen boxes were stacked in a carton box (49.5×27×19.2 cm)
and kept in a −18 °C freezer on the vessel for 2 wk. They were offloaded
in Dutch Harbor, AK and placed in a thermo-corrugated box insulated
with Styrofoam before being airfreighted to Seattle. Upon arrival, the
thermo-boxes were wrapped in a thermo-blanket and transported to
the OSU Seafood Laboratory (Astoria, OR, USA). Upon arrival, the core
temperature of fish was to be determined −16 °C and fish were kept at
−25 °C until thawing