The strawberry vinegar biofilm was plated by triplicate streaking
the loop with three samples of approximately 100 mm2 onto
the GY medium (5% glucose, 1% yeast extract, and 1.5% agar) supplemented
with natamycin (100 mg/L) (Delvocid, DSM; Delft, The
Netherlands) to suppress fungal growth. After 2 days of incubation
at 28 C, the colonies were grown on the GYC medium (10% glucose,
1% yeast extract, 2% CaCO3, and 1.5% agar) to determine their acid
production by the dissolution of CaCO3 precipitates on plates. The
colonies with a clear halo around them were subjected to the
catalase test; positive results supported their putative identity as
AAB and were identified by molecular methods