Based on results of RAPD-PCR and size of the inhibition zone,
isolate ST71KS was selected for future studies. The microorganism
was identified using PCR species-specific primers (Torriani et al.,
2001) and further confirmed by amplification of 16S rDNA with
primers F8 and R1512 (Felske et al., 1997). The amplified fragments
were purified (QIAquick PCR Purification Kit e Qiagen, Hilden,
Germany), sequenced, and compared to sequences available at
GenBank using BLAST (Basic Local Alignment Search Tool).
Based on results of RAPD-PCR and size of the inhibition zone,isolate ST71KS was selected for future studies. The microorganismwas identified using PCR species-specific primers (Torriani et al.,2001) and further confirmed by amplification of 16S rDNA withprimers F8 and R1512 (Felske et al., 1997). The amplified fragmentswere purified (QIAquick PCR Purification Kit e Qiagen, Hilden,Germany), sequenced, and compared to sequences available atGenBank using BLAST (Basic Local Alignment Search Tool).
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