Cotton fibers were infused with about 3000 mg/kg of silver nanoparticles (12 ± 3 nm in diameter) in the entire volume of the fiber and underwent consecutive launderings up to 50 cycles in water and a detergent solution. Colorimetric, spectroscopic, and elemental analyses on the silver nanoparticle infused cotton fabric consistently showed the two-step silver release in water and a detergent solution with increasing laundering cycles. The rapid release during the initiallaundering, which was comparable to those from surface treated commercial and laboratory-prepared textiles in the literature, was attributed to be from the external nanoparticles formed on the fiber surface.