Aqueous extracts of medicinal herbs were tested for activity against Enterococcus
faecalis, Actinomyces viscosus, Streptococcus salivarius, Streptococcus mutans, and Streptococcus
sanguis grown in brain heart infusion (BHI) broth. A broth microdilution assay was used to
determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration
(MBC). A disk diffusion assay was performed by inoculating bacterial cultures on BHI
agar plates with paper disks soaked in each of the medicinal herb extracts. Inhibition of the
synthesis of water-insoluble glucans by S. mutans was also investigated