After the cell mass obtained,
the fermentation tests were carried out in sugar cane juice at 15◦
Brix, at temperature of 28 ◦C, without cells recycling. In this first
step 88 yeast strains were analyzed and only 19 were selected,
according to their growth and ethanol concentration. In the second
step the number of cells was standardized to 108 cells/mL and
a new fermentation tests were started in a feed batch process with
cells recycling and sugar concentration at 18◦ Brix, temperature of
28 ◦C. The tests were carried out feed batch process with recycles of
yeast cells and 12 hour of fermentation time. Ethanol content, pH,
wet and dry cell mass, cell viability, ethanol yield and fermentation
efficiency were evaluated in the final fermentation time.