The experimental animals were anaesthetized
using lignocaine 2% injections,
over the local selected region.
The rats were depilated over the region excision wound was infected by cutting a way of 5mm square thickness of skin from the predetermine area,
the wound was left and rest to the open environment then the drugs reference standard (0.01 % w/w gentamycin ointment) control (simple ointment BP) only Mimosa pudica extract both shoot & root in Methanol extract is applied.
(10 %w/w simple ointment) were applied till the wound was healed.
This model was used to monitor the wound contraction and wound closer time.
Wound contraction was calculated as % reduction in wound area.
The progressive change in wound area is monitored by calculating the decreasing area.