In order to demonstrate that the strain could solubilize cal-
decrease in pH, a phytin agar plate test and an activity assay
of phytase and phosphatase were conducted. As shown
in Fig. 2-A, a transparent zone appeared on screening
agar medium containing calcium phytate after inoculated
with T. asperellum Q1 at 28°C for 3 d. Meanwhile, nothing
appeared on the agar plate without T. asperellum Q1.
Furthermore, activities of phytase and phosphatases in
of time after inoculation with T. asperellum Q1 comparing
to uninoculated control. The highest phytase activity was
up to 0.17 U mL–1 (Fig. 2-B) on the 3rd day after inoculating
T. asperellum Q1. The maximum of acid phosphatase activity
was found to be 7.15×10–3 U mL–1 after 2 d of incubation
whereas the alkaline phosphatase activity was much lower
than that of acid phosphatase (Fig. 2-C). Therefore, all the
results from in vitro phosphate solubilizing test, phytin agar
plate test and activity assay of phytase and phosphatase
proved that metabolites (mainly enzymes) of T. asperellum
Q1 could hydrolyze unsoluble organic phosphate into the
available phosphorus that was absorbed by strain Q1.