1.Cut out the gene.
2. insert gene into a vector with a selectable antibiotic resistance marker gene.
3. copy vector in bacteria
4. cost tungsten or gold particles with DNA vector.
5. load vector-coated particles onto teflon bullet.
6. load bullet into gene gun
7. shooting the gene gun releases the particles at a high velocity penetrating the plant cell.
8. the vector enters the cell. The genes are incorporated into the plant gonome.
9. the cell are plated on a selective antibiotic media. Only cells that have incorporated the vector will grow.
10.These cell are transferred to medium containing plant growth factors.