Wild Centella asiatica was collected from the
Jabra, Manikgang. The plant was identified and
authenticated by Department of Botany
Jahangirnagar University, Savar, Dhaka
(NoJUBD: 1206). The fresh leaves with petioles
were then air dried in shadow and grinded by
mechanical grinder. Fine plant powder was then
used for the exhausted extraction by Soxhlet
apparatus for four repeated cycle using 100%
ethanol, 50% ethanol and water as extraction
solvent. During extraction solute-solvent ratio
was 10:1 and extraction temperature was
45º±2ºC. The extracts were then filtered,
evaporated using oven to a thick residue at 45°C
and stored at 4°C. This crude extract was used for
further analyses.
2.2 Total Polyphenol