shows the stability of the covalently immobilized preparations compared to the crude lipases in the presence of methanol. As with the thermal stability, the differences are evident. Liquid CALB retained 75% of residual activity in 90% methanol after 24 h incubation at 25 ◦C. Even better, covalently immobilized CALB showed excellent stability with increasing concentrations of methanol. So CALB seems to be the most methanol stable enzyme out of the three. However, both forms of TLL were quite stable in up to 30% of enzyme and decreased FAME yields [7]. For biodiesel synthesis, at least a stoichiometric amount of methanol is required for the complete conversion of triacylglycerols (TAGs) to their corresponding FAMEs. However methanolysis is decreased significantly by adding more than 1/2 molar equivalent of methanol at the beginning of the enzymatic process. This inactivation caused by the polar short chain alcohols has been a major obstacle for enzymatic biodiesel production