At selected times, cheese samples were homogenized in peptone water (1:10) and dilution drops (20 ml) were spotted in duplicate onto the adequate media to determine the number of
CFU/ml of each microorganism. Yeasts and moulds were enumerated on plates containing yeast selective agar YGC (Biokar Diagnostic, France) incubated at 28 C for 48e72 h. Psychrotrophic bacteria were determined using plate count agar (PCA, Biokar Di agnostics, France) after incubation for 5 days at 7 ± 1 C. Listeria spp. was enumerated on plates containing the L. innocua selective agar Oxford (Biokar Diagnostics, France) incubated at 37 C for 48 h. Enumeration of colonies was performed, and microorganism growth was expressed as log CFU/ml. Determinations were performed in duplicate in two separate experimental runs.
At selected times, cheese samples were homogenized in peptone water (1:10) and dilution drops (20 ml) were spotted in duplicate onto the adequate media to determine the number ofCFU/ml of each microorganism. Yeasts and moulds were enumerated on plates containing yeast selective agar YGC (Biokar Diagnostic, France) incubated at 28 C for 48e72 h. Psychrotrophic bacteria were determined using plate count agar (PCA, Biokar Di agnostics, France) after incubation for 5 days at 7 ± 1 C. Listeria spp. was enumerated on plates containing the L. innocua selective agar Oxford (Biokar Diagnostics, France) incubated at 37 C for 48 h. Enumeration of colonies was performed, and microorganism growth was expressed as log CFU/ml. Determinations were performed in duplicate in two separate experimental runs.
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