Given that alterations in cellgrowth, physiology and metabolic activity may be induced by cellimmobilization (e.g. effects on the energetic metabolism activationand targeted protein expression to support it, increased substrateuptake and product yield, increased tolerance against inhibitorycompounds, increased hydrolytic enzyme activities, etc.) [8], it ispossible that the promotional activities of TC on maltose fermen-tation are connected to the activity of enzymes and transportersinvolved in the process. Therefore, in order to develop a technol-ogy for maltose fermentation at extremely low temperatures usingimmobilized yeast, the strategy adopted was to study the effect ofTC on the fermentation of maltose and glucose synthetic media andto compare the results with those using free yeast cells.