The infectious salmon anaemia virus isolate used in this study was a
Chilean field isolate CH0108 (accession # EU851083). The virus was
obtained from Atlantic salmon tissue sent from Chile to Norway on
dry ice, isolated in ASK-cells and stored in liquid nitrogen. This ISA
virus isolate has been described previously (Lauscher et al., 2011; Vike
et al., 2009;Wolf et al., 2013). Fishwere intraperitoneally (i.p.) infected
with 0.1 mL of 3 × 106 virus per mL measured using TCID50. Ten of
these ISA positive fish were used further in this study post mortem.