In vitro P. delenatii shoot elongation in the darkness
To investigate effect of the darkness on shoot elongation, 5-leaf young shoots were cultured on SH medium supplemented with 0.5 mg l-1 NAA, 0.5 mg l-1 BA, 30 g l-1 sucrose, 9.0 g l-1 agar, and 1.0 g l-1 AC, and placed in the darkness without subculture (Fig. 1g1). The shoot length was measured after 1–4 months of culture.
In vitro P. delenatii shoot elongation in the darknessTo investigate effect of the darkness on shoot elongation, 5-leaf young shoots were cultured on SH medium supplemented with 0.5 mg l-1 NAA, 0.5 mg l-1 BA, 30 g l-1 sucrose, 9.0 g l-1 agar, and 1.0 g l-1 AC, and placed in the darkness without subculture (Fig. 1g1). The shoot length was measured after 1–4 months of culture.
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