MATERIALS AND METHODS
Plant Extract
Seeds of M. siamensis were dried at 50–60 °C for 72 hours and ground. 100 g of
seedpowder was extracted with 250 ml of dichloromethane and evaporated by rotary
evaporator to obtain the crude extract (Diagram 1).
Test for Insecticidal Activity
The tested insects, house flies were propagated and fed at every stage i.e. egg,
maggot, pupae and adult at the Department of Biology, Faculty of Science, Chiang Mai
University.
Different compounds were separated from the crude extract of M. siamensis by
thin layer chromatography (TLC). 0.25 g of different bands of the compounds on TLC
were crushed, dissolved in 5 ml of 10 % acetone and sprayed on all of the studied stages
of the house fly.
The mortality of egg and pupae were observed when 60 % of the control had
developed to the next stage. Data of the maggots were observed 1 day after the