I3S Pattern is the tool for species with hard to annotate markings. This may mean markings which cannot be annotated using spot centers or ellipses. Pattern may also be suitable for species with lots of small spots making it difficult to choose about 30 spots consistently. Pattern only requires you to identify the three reference points, and the general identification area. After this, the relevant key points are extracted automatically. Each key point has a size and location, denoted by a circle with varying diameter.