Micropropagation of Aglaonema var. Cochin was performed using axillary shoots as explants. Shoots could be induced on the bulb region on Murashige and Skoog (MS) medium supplemented with 1.5 mg/l thidiazuron (TDZ). For shoot proliferation, small shoots were multiplied on MS medium supplemented by 1.5 mg/l TDZ and 3 mg/l benzylamino purine (BAP), whose addition was important to avoid callus growth. The highest multiplication rate of Aglaonema shoots was achieved at the 5th subculture. After the 5th subculture (10 weeks) 1000 shoots could be obtained from two initial axillary shoots. Plantlets rooted and developed on MS medium containing 3 mg/l indole-3butyric acid. Acclimatization was performed successfully with 100% survival rate on sphagnum moss followed by transfer to soil.