Campylobacter is the most common food-borne pathogen in developed and developing countries. Poultry is an importance vehicle for Campylobacter infection in humans. Due to a short time required for analysis of food Products, more rapid, sensitive and specific methods have been developed for Campylobacter detection. The by using methods multiplex PCR (mPCR) to detect Campylobacter jejuni and C. coli in duck each age and environment within the farm Includes samples from slaughterhouses. That samples have all 1,080 isolate It was found that as the bacteria Campylobacter 212 isolate (19.62%) for their suitability to detect Campylobacter jejuni and C. coli. I found C. jejuni 33 isolate () and C. coli 179 isolate ().Then selected C. jejuni come split Subspecies. The by using methods Multilocus sequence typing (MLST) to study the changes of gene genetic 7 position in housekeeping genes to examine the relationship between genetic variants that are alike or different. By analyzing the relationship by building Phylogenetic tree compared to other sources of C. jejuni in the database GenBank.