SDS-PAGE
The protein patterns of shrimp treated with different types of
saccharides are shown in Figure 4. The electrophoretic profiles of
muscle revealed a broad band at approximately 200 kDa, which
was assigned to the myosin heavy chain (MHC). Paramyosin
(approximately 90 kDa) was also present, as well as actin (approximately
40 kDa). Additional bands corresponded to tropomysin
(approximately 37 kDa), and myosin light chain (MLC; approximately
20 kDa) (Gomez-Estaca and others 2014). There was a ´
considerable decrease in the intensity of MHC, paramyosin, actin,
and MLC in the control samples (Figure 4E), compared with
saccharide-treated samples (Figure 4A to D) after 6 wk of storage.
The MHC and MLC bands began to significantly disappear
in samples without added cryoprotective saccharides. This