Briefly, contents of the positive guts were placed in 2.5 per cent potassium
dichromate (K2Cr2O7) solution and incubated at 37C temperature
and 60–80% humidity for 60–72 h. The sporulation of the oocysts
was confirmed by taking a drop of suspension on glass slide and
examining the sporocysts containing sporozoites under the
microscope (40X).