2.4. Quantitative determination of the phytochemical constituents in samples Aluminum chloride colorimetric method was used for flavonoid determination[15] while total phenol content of the extracts was determined using the method reported by Singleton et al[16]. The method of Oloyed[17] was used to determine the amount of alkaloids and saponins in the mushroom extracts while tannin content was quantified with the method described by AOAC[18].