Material and methods
Collection the samples and isolation of microalgae
Field work for taking samples was done by collecting the
waters from seashore, salt farm, pond, lake at Lombok Island
(West Nusa Tenggara) and Giam Siak Kecil (Riau Province).
Collection was also done by picking up the sample using cotton
stick thereafter soaking in the enrichment or selected
sterile media. The other method was done by collecting and
filtering the water samples, therefore, it's concentrated using
vacuum pump with 45 mmfilter paper pore size. Concentrated
water containing algae were transferred to enrichment medium.
Meanwhile, the filter paper was cut and also soaking
into enrichment or selected sterile media directly, following
Sekiguchi's protocol [10]. Further procedures for isolation,
characterization and screening were done at laboratory.
Microalgae were isolated manually by seduce the single algal
cell using micropipeting technique under the light inverted
microscope with 20 objective lens (Olympus CKX41). The
expected algal single cell was transferred several times into
micro-plates well for purification, following Sekiguchi's protocol
[11].
Material and methodsCollection the samples and isolation of microalgaeField work for taking samples was done by collecting thewaters from seashore, salt farm, pond, lake at Lombok Island(West Nusa Tenggara) and Giam Siak Kecil (Riau Province).Collection was also done by picking up the sample using cottonstick thereafter soaking in the enrichment or selectedsterile media. The other method was done by collecting andfiltering the water samples, therefore, it's concentrated usingvacuum pump with 45 mmfilter paper pore size. Concentratedwater containing algae were transferred to enrichment medium.Meanwhile, the filter paper was cut and also soakinginto enrichment or selected sterile media directly, followingSekiguchi's protocol [10]. Further procedures for isolation,characterization and screening were done at laboratory.Microalgae were isolated manually by seduce the single algalcell using micropipeting technique under the light invertedmicroscope with 20 objective lens (Olympus CKX41). Theexpected algal single cell was transferred several times intomicro-plates well for purification, following Sekiguchi's protocol[11].
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