Based on the results, MK-7 concentration increased between cell's mid-exponential growth and the stationary phase. The 80% of total MK-7 concentration was produced after 30 h of fermentation where the concentration of glycerol as a carbon source became exhausted. This research is the first report on its own which significantly contributes to the initial development of a novel functional dairy product high in MK-7. These results provide a basis of knowledge and new discoveries that can be carried out to develop a product that can act as a preventative in health issues such as osteoporosis and cardiovascular diseases.