The present research work explores bio diesel production from one of the novel non-edible feed stocks viz.Cyprus carpi fish oil. Production of bio diesel from this oil was successfully performed through base-catalyzed transmogrification assisted by a co-solvent. Hexane and potassium hydroxide (KOH) were chosen as the co-solvent and the catalyst, respectively. The experimental parameters included in the optimization process were type of co-solvent, type and concentration of the base catalyst, methanol to oil molar ratio, hexane to methanol volume ratio, temperature and time. The maximum yield of biodiversity C. carpi fish oil (98.55 ± 1.02% _ 97.24% w/w ester content) was obtained at 0.60% KOH w/w, 5:1 methanol to oil molar ratio, 1.5:1 hexane to methanol volume ratio, 50 _C reaction temperature and 30 min of reaction. The Fourier Transform Infrared spectroscopy and thin layer chromatography we reused to ensure the conversion of fish oil into bio diesel. The bio diesel properties were within the recommended bio diesel standards as prescribed by ASTM D 6751 and EN 14214. The most remarkable features of C. carpi fish oil bio diesel are the flash point and viscosity which are close to those of petrol diesel.Moreover, blends of bio diesel and petrol diesel were complied with the limits prescribed in the ASTMD 7467 standards. Hence, the oil is a potential non-edible feed stock for bio diesel production. Cosolventtranse stratification of C. carpi fish oil was found to follow first order kinetics and the activation energy was calculated to be 32.46 kJ/mol. However, it was concluded that the use of co-solvent within the reaction medium enhanced yield and properties of bio diesel comparing with that produced via non solvent process.