Also, it was pointed out that mobile phases containing tetrahy-drofuran, sulfuric acid and hydrochloric acid led to the growthinhibition of test bacteria on the plates in case of B. s. and Micro-cococcus luteus [19]. Especially, acidic mobile phases destroyedthe bioassay response, but at the same time, were essential forthe separation. Despite all these efforts and due to its limitations,there is need for a more streamlined, universally applicable work-flow, for less diffuse antibiotic zones and for a more homogeneousbackground of the bioautogram. Such improvements would evenallow a reliable quantitation of the discovered antibiotics. In thisstudy, such a streamlined workflow was demonstrated producinga homogenous background of the bioautogram and thus baseline ofthe biodensitogram. Different growth media were compared withregard to detectability. The challenge of the use of an acidic mobilephase in DB was overcome. The latter is of special interest,