2.2. Supercritical CO2 extraction
Experiments were carried out in a semi-continuous supercritical CO2 extractor used in previous work (Machmudah et al., 2008a). The apparatus was equipped with an extractor (10 cm of length, 2 cm of inside diameter, and 10 ml of capacity) (Thar Tech, Inc., USA); a back pressure regulator (SCF-BPG, Jasco, Japan); a chiller (Cooling Unit CLU-33, Iwaki Asahi Techno Glass, Japan); a high pressure pump for CO2 (Intelligent Prep. Pump. PU-2086 Plus,Jasco, Japan); collection vials; and a wet gas meter (Sinagawa Co.,Japan).