One milliliter of carrot juice was extracted with 10 mL of methanol
and agitated in a darkened area for 24 h in an orbital shaker
(L.E.D. Model 3518. Lab-Line Instruments, Inc., Melrose Park, IL.).
After centrifugation at 4500 rpm for 10 min in a MSE Centaur 2
centrifuge (MSE UK Ltd., London, UK), supernatants were collected,
stored at 4 C and used to quantify phenolic compounds and antioxidant
capacity