The effect of the extract on the 2,2-diphenyl-1-picrylhydrazyl
(DPPH) radical was estimated according to the procedure described
by Brand-Williams, Cuvelier, and Berset (1995). The extract (0.1 ml)
was added to 3.9 ml of DPPH radical 6 105 Min methanol which
was prepared daily. The decrease in absorbance was determined at
515 nmafter incubation for 30 min. ADPPHradical solution without
sample was used as a control and the DPPH percentage inhibition
was calculated according to the following equation: