2. Materials and method
2.1. Raw materials and Chemicals
Fish Scales were taken from the local fish market for this project. Acid (HCL, 36.5g/mol) 1% solution of HCL was prepared for demineralization. Sodium hydroxide (NaOH, 40g/mol) 0.5 N solution of NaOH was prepared for Deproteinization. pH meter and distilled water were also used in the experimental process. All chemicals used were of laboratory grade.