2. Materials and methods
2.1. Animals
Embryo transfer donors and recipients (cows between 2
and 5 years of age) were provided from a specific pathogenfree
herd free from BVDV and bovine herpesvirus. These
animals were housed in remote pathogen-free facilities
with a specially designated animal care crew and were
tested multiple times using virus isolation and seroneutralization
to ensure BVDV-free status.