Effect of polymer loading on microalgal cell disruption
Microalgal cell disruption was increased when treated with polymers with increasing amine content. However, because pNcAA-5 and pNcAA-10 did not have a significant difference on cell disruption (section), pNcAA-5 was used to study the influence of polymer loading on cell disruption with and without enzyme. It should be noted that using lower amine content in the copolymer is advantageous for the purpose of polymer recovery, because highly charged polymers exhibit poor phase separation above the LCST. As shown in fig. 6, cell disruption with polymer only increased from about 3 to 32% with the increase of polymer loading from 0 to 1 g/g BDW. However, polymer loading above 0.1 g/g BDW did not provide a significant increase of cell disruption. When the polymer was used to aid enzymatic cell disruption, the polymer loading can be reduced to 0.05 g/g BDW which resulted in about 57% cell disruption, similar to 0.1 g/g BDW and hiher polymer doses. In addition, enzyme and 0.01 g/g BDW polymer achieve approx. 32% cell disruption which was close to the highest cell disruption that resulted from polymer solutions with dosages higher than 0.2% g/g BDW. At some polymer composition, the cell disruption efficiency levels off because the microalgal cell surface becomes saturated by polymer, which occurs around 0.05-0.1 g/g BDW loading rate. The microalga size of C. is 5-20 [24] is much larger than polymers dissolved in aqueous solutions; therefore. A single cell could absorb multiple amine groups from different polymers. Therefore, many polymers from solution are likely contributing to the disruption of a single cell, which could significantly reduce the polymer dosage efficiency (disrupted cells/g polymer). However, the lower bound of polymer composition in solution will be limited by the phase behavior of the polymer. In order to collect the polymer after use, the solution must be slightly heated resulting in precipitation of the copolymers. Solutions below 2 wt.-% polymer do not precipitate from solution efficiently for recover and reuse [25].
ผลของพอลิเมอร์ที่โหลดบนทรัพยเซลล์ microalgal Microalgal cell disruption was increased when treated with polymers with increasing amine content. However, because pNcAA-5 and pNcAA-10 did not have a significant difference on cell disruption (section), pNcAA-5 was used to study the influence of polymer loading on cell disruption with and without enzyme. It should be noted that using lower amine content in the copolymer is advantageous for the purpose of polymer recovery, because highly charged polymers exhibit poor phase separation above the LCST. As shown in fig. 6, cell disruption with polymer only increased from about 3 to 32% with the increase of polymer loading from 0 to 1 g/g BDW. However, polymer loading above 0.1 g/g BDW did not provide a significant increase of cell disruption. When the polymer was used to aid enzymatic cell disruption, the polymer loading can be reduced to 0.05 g/g BDW which resulted in about 57% cell disruption, similar to 0.1 g/g BDW and hiher polymer doses. In addition, enzyme and 0.01 g/g BDW polymer achieve approx. 32% cell disruption which was close to the highest cell disruption that resulted from polymer solutions with dosages higher than 0.2% g/g BDW. At some polymer composition, the cell disruption efficiency levels off because the microalgal cell surface becomes saturated by polymer, which occurs around 0.05-0.1 g/g BDW loading rate. The microalga size of C. is 5-20 [24] is much larger than polymers dissolved in aqueous solutions; therefore. A single cell could absorb multiple amine groups from different polymers. Therefore, many polymers from solution are likely contributing to the disruption of a single cell, which could significantly reduce the polymer dosage efficiency (disrupted cells/g polymer). However, the lower bound of polymer composition in solution will be limited by the phase behavior of the polymer. In order to collect the polymer after use, the solution must be slightly heated resulting in precipitation of the copolymers. Solutions below 2 wt.-% polymer do not precipitate from solution efficiently for recover and reuse [25].
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