In this study, bioethanol was sequentially produced using the lipid-extracted residual biomass of Chlorella sp. KR-1 asfermentation feedstock. The lipid-extracted residual biomass was directly saccharified and fermented with high efficiency since the cell wall was disrupted during lipid extraction step in biodiesel production process. In another manner, the saccharification broth generated by enzymatic method was directly used for bioethanol
fermentation without any pretreatment. The waste residual biomass generated from biodiesel production process could be
used as feedstock for bioethanol fermentation, which contributes to economic feasibility of biofuel production from microalgae.