2.3. Total protein concentration determination
Total protein concentrations were determined by Bradford’s
method using Bovine Serum Albumin (BSA) as the protein
standard.12
2.4. Starch hydrolyzed assay
a-Amylase activity was determined by measuring the formation
of reducing sugars released during starch hydrolysis. The
amount of liberated reducing sugar was determined by Dinitrosalicylic
acid (DNS) method. Glucose was used to construct
the standard curve