then has been published previously. Most studies used excision of
spots on 2D-gels, followed by mass-spectrometry e.g. [5,8,9]. With
this 2D-gel method only excised spots are analyzed. With our 1Dgel
method, however, we analyzed the whole gel lane and did,
thus, not rely on visible protein staining. In addition, our 1D-gel
method is more suitable for analyzing membrane proteins, which
are ubiquitous in MFGM [10]. The same 1D-gel method, was
recently used for studying the proteome of bovine milk serum [11]
and bovine MFGM