electrophoresis experiments in order to confirm it.
In this way, the spectroscopic and electrophoresis
analysis of the fraction II (B-PE) obtained from
the large-scale chromatography process was carried
out (Fig. 6). Electrophoresis analysis shows
that fraction II yields three bands corresponding
to B-PE subunits: , , (Fig. 6A). By comparison
with standards, we obtained molecular mass
of the , and -subunits of about 16 500, 18 000
and 27 000 Da, respectively. Fig. 6B shows visible
absorption, fluorescence emission and anisotropy
spectra of purified B-PE. Absorbance maximum is
545 nm, whereas the fluorescence emission maximum
is 574 nm and the anisotropy spectrum is
similar to those earlier published for hexameric
phycoerythrins from other red algae (MacColl,
1991; Guard-Friar et al., 1989).
electrophoresis experiments in order to confirm it.
In this way, the spectroscopic and electrophoresis
analysis of the fraction II (B-PE) obtained from
the large-scale chromatography process was carried
out (Fig. 6). Electrophoresis analysis shows
that fraction II yields three bands corresponding
to B-PE subunits: , , (Fig. 6A). By comparison
with standards, we obtained molecular mass
of the , and -subunits of about 16 500, 18 000
and 27 000 Da, respectively. Fig. 6B shows visible
absorption, fluorescence emission and anisotropy
spectra of purified B-PE. Absorbance maximum is
545 nm, whereas the fluorescence emission maximum
is 574 nm and the anisotropy spectrum is
similar to those earlier published for hexameric
phycoerythrins from other red algae (MacColl,
1991; Guard-Friar et al., 1989).
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