3.2. H2O2 scavenging of HPOX nanoparticles
Either HPOX nanoparticles, POX nanoparticles or HBA was
added into 1 mL of H2O2 solution (10.0 mM) and the H2O2
concentrationwas measured after 24 h of incubation at 37 C using
the Amplex Red assay. Fig. 2 illustrates the reduction of H2O2
concentration by HBA, POX nanoparticles or HPOX nanoparticles.
POX nanoparticles exhibited a slight reduction of H2O2 concentration
in a dose-dependant manner. HBA showed minimal reduction
of H2O2 concentration. However, HPOX nanoparticles showed
a significantly lower H2O2 concentration than POX nanoparticles
and HBA. More than 50% of reduction of H2O2 concentration was
observed with 1 mg of HPOX nanoparticles, demonstrating that
HPOX nanoparticles quench H2O2 in a dose-dependent manner.
HPOX nanoparticles showed a more dose-dependant H2O2 scavenging
activity than POX nanoparticles and HBA.