Total phenolic content of rice bran was measured according to the method of Dewanto and others. Folin-ciocalteu’sphenol reagent (0.2 mL) was added to each sample (0.1 mL),
and left to stand for 1 min at room temperature. Then 3 mL 5% Na2 CO3 was added to mixture, and left to stand for 1 h in dark place. The absorbance was measured at 760 nm using spectrophotometer (UV-1650PC, Shimadzu Co., Kyoto, Japan). Gallic acid was used as a standard, and total phenolic contents were expressed as gallic acid equivalents.Total flavonoid content of rice bran was measured according to the method of Jia and others (1999). Each sample (500 μL) was mixed with 5% NaNO2 (75 μL), and then left to stand for 5 min at room temperature. The mixture was sequentially mixed with 150 μL 10% AlCl3 , 500 μL 1 M NaOH, and 275 μL distilled water. The absorbance was measured at 510 nm using spectropho- tometer. Catechin was used as a standard, and total flavonoid con- tents were expressed as catechin equivalents
Total phenolic content of rice bran was measured according to the method of Dewanto and others. Folin-ciocalteu’sphenol reagent (0.2 mL) was added to each sample (0.1 mL),and left to stand for 1 min at room temperature. Then 3 mL 5% Na2 CO3 was added to mixture, and left to stand for 1 h in dark place. The absorbance was measured at 760 nm using spectrophotometer (UV-1650PC, Shimadzu Co., Kyoto, Japan). Gallic acid was used as a standard, and total phenolic contents were expressed as gallic acid equivalents.Total flavonoid content of rice bran was measured according to the method of Jia and others (1999). Each sample (500 μL) was mixed with 5% NaNO2 (75 μL), and then left to stand for 5 min at room temperature. The mixture was sequentially mixed with 150 μL 10% AlCl3 , 500 μL 1 M NaOH, and 275 μL distilled water. The absorbance was measured at 510 nm using spectropho- tometer. Catechin was used as a standard, and total flavonoid con- tents were expressed as catechin equivalents
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