Utilization of thermotolerant microorganisms enables fermen- tation to be performed at high temperatures, which have several advantages such as reduce cost for cooling system, reduce contam- ination of mesophilic microorganisms and increase the speed of catalytic reactions related to fermentation. In this work, isolation andcharacterizationofthermotolerantyeastscapableofproducing ethanol from Jerusalem artichoke (Helianthus tuberosus L.),