Enzyme-Linked Immunosorbent Assay (ELISA) is biochemical assay technique used mainly in immunology.
Quantitative immunological procedures in with the Ag-Ab reaction is monitored by enzyme measurements.
The term ELISA was first used by Engvall and Perlma in 1971
The ELISA test, or enzyme immunoassay (EIA) , was the first screening test commonly employed for HIV. It has a high sensitivity.