The assay for antagonism was performed on potato
dextrose agar (PDA) adopting dual culture method as
suggested by Zivkovic et al. [19]. A 5 mm of mycelial
agar disc from test fungal pathogen cultures was placed
on the one side of a Petri plate containing PDA medium.
The plates were then incubated at 250C for 24 hrs. A
loopful of test antagonistic bacterial culture was then
streaked 3 cm away from the disc of pathogen on the
same dish. PDA plates inoculated only with pathogens
were maintained as control. The zone of inhibition was
recorded as the distance between the fungal pathogen
and the area of antagonist growth after 7 days. The
experiment was performed in three replications