A blastP query using the predicted amino acid sequence of the βLR16 upstream gene product
showed the highest identity with a hypothetical protein from the unclassified Zetaproteobacterium
SCGC AB-137-C09 (WP_018282215.1), at 39% identity over 632 amino acids. This
βLR16 protein showed evidence of an N-terminal urea transport membrane domain, and a
peptidase family M23 domain (zinc metallopeptidase). A blastP query using the predicted
amino acid sequence of the βLR16 downstream gene product showed a putative transcriptional
regulator from the Betaproteobacteria Pseudogulbenkiania ferrooxidans (WP_008955746.1) to
have the highest identity, with 56% identity over 228 amino acids. This βLR16 protein is predicted
to encode an N-terminal response regulator receiver domain (Conserved Domain
cd00156) and a C-terminal effector domain (Conserved Domain cd00383) [25], and can be
Metagenomic Response Regulator Confers Antibiotic Resistance
PLOS ONE