In this work, aqueous extraction of rice bran oil was done without and with ultrasound pretreatment.Key factors controlling the extraction and optimal operating conditions were identified.The highest extraction efficiency was found at pH = 12, temperature of 45 °C, agitation speed of 800 rpm and agitation time of 15 min, ultrasound treatment time of 70 min and ultrasound treatment temperature of 25 °C. Moreover, extraction yields were compared to ultrasound-assisted aqueous extraction and Soxhlet extraction